ProductCategory:Pre-maderecombinantAAV;AAV-CAG-DIO-ChIEF-tdTOMATO;AAV1-CAG-DIO-ChIEF-tdTOMATO
Description:
AAV1-CAG-DIO-ChIEF-tdTOMATOisapre-maderAAVinSEROtype1whichwillCredependentlyover-expressDIO-ChIEF-tdTOMATOdrivenunderCAGpromoter(alsoknownasCBApromoter)asillustratedasthecartoonbelow.
ChIEFisahybridofchannelrhodopsin1and2withadditionalL170Imutation(ChR1numbering)oratL131I(ChR2-numbering),leADIngtolargecurrentsinoocytesandHEK-cellsandalmostwildtypelikeopenstatelifetime.ChIEFfunctionaslight-gatedionchannelsandveryusefulformanybioengineeringandneuroscienceapplicationssuchasphotostimulationofneuronsforprobingofneuralcircuits.UsingtdTOMATOtaggedChIEF,light-stimulatedaxonsandsynapsescanbeidentifiedinintactbraintissue.Thisisusefultostudythemoleculareventsduringtheinductionofsynapticplasticity.ChIEFhasalsobeenusedtomaplong-rangeconnectionsfromonesideofthebraintotheother,andtomapthespatiallocationofspecificinputsonthedendritictreeofindividualneurons.
IntheDIOscenario,thetransgeneofinterestisinsertedinreverseorientationrelativetothe5"promoterandisflankedbyoppositelyorientedloxPandlox2272sites. IntheabsenceofCreexpression,thetransgenewillnotbeproduced.InthepresenceofCreexpression,thetransgenewillbe"Flip-exchanged"orFLEXed,leadingtotheexpressionofthetransgene.ThisisduetoapermanentCre-mediatedrecombination/inversionoftheflankedtransgene.ThisarrangementiscalledDIO(double-floxedinverseORF),Cre-ON,Flex-rev(reverse),Flex-ON/FlexON,orDIO-AAV/AAV-DIO(double-floxedinverseORFinAAV). 
Titer:>1x1013VG/mL
Size:>30µL(StdPack)
StorageBuffer:SterilePBS
InfectionProtocol:
VectorMap:
